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arpc2 goat polyclonal  (Abnova)

 
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    Structured Review

    Abnova arpc2 goat polyclonal
    Homology and function of selected antigens.
    Arpc2 Goat Polyclonal, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arpc2 goat polyclonal/product/Abnova
    Average 90 stars, based on 1 article reviews
    arpc2 goat polyclonal - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients"

    Article Title: Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients

    Journal: NPJ Breast Cancer

    doi: 10.1038/s41523-021-00257-1

    Homology and function of selected antigens.
    Figure Legend Snippet: Homology and function of selected antigens.

    Techniques Used: Migration, Inhibition

    Four pooled siRNA knocked down expression of the targets VPS35 ( a , b ), ARPC2 ( c , d ), SERBP1 ( e , f ), KRT8 ( g , h ), and Pdia6 ( i , j ). Decreased expression of these proteins demonstrate decreased survival ( a , c , e , g , and i ) and increased apoptosis ( b , d , f , h , and j ) as compared to mock transfected in either triple negative (TNBC, HCC70) or HER2 positive (HER2, SKBR3) human breast cancer cell lines. Survival is measured as % survival compared to mock transfected cells and apoptosis is measured as fold increase compared to mock transfected calls. All studies are in triplicate. Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
    Figure Legend Snippet: Four pooled siRNA knocked down expression of the targets VPS35 ( a , b ), ARPC2 ( c , d ), SERBP1 ( e , f ), KRT8 ( g , h ), and Pdia6 ( i , j ). Decreased expression of these proteins demonstrate decreased survival ( a , c , e , g , and i ) and increased apoptosis ( b , d , f , h , and j ) as compared to mock transfected in either triple negative (TNBC, HCC70) or HER2 positive (HER2, SKBR3) human breast cancer cell lines. Survival is measured as % survival compared to mock transfected cells and apoptosis is measured as fold increase compared to mock transfected calls. All studies are in triplicate. Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

    Techniques Used: Expressing, Transfection

    Percent decreased tumor growth from TgMMTV-neu (light gray) and C3(1)Tag mice (dark gray) n = 8/group as compared to control PBS vaccinated mice (white). Mice were vaccinated with peptides from VPS35, ARPC2, SERBP1, KRT8, and PDIA6 or PBS with CFA/IFA adjuvant ~14 days for three times and then with 3 × 10 5 syngeneic mouse mammary tumor cells (MMC for TgMMTV-neu and M6 for C3(1)Tag) implanted. **** p < 0.0001.
    Figure Legend Snippet: Percent decreased tumor growth from TgMMTV-neu (light gray) and C3(1)Tag mice (dark gray) n = 8/group as compared to control PBS vaccinated mice (white). Mice were vaccinated with peptides from VPS35, ARPC2, SERBP1, KRT8, and PDIA6 or PBS with CFA/IFA adjuvant ~14 days for three times and then with 3 × 10 5 syngeneic mouse mammary tumor cells (MMC for TgMMTV-neu and M6 for C3(1)Tag) implanted. **** p < 0.0001.

    Techniques Used: Control, Adjuvant

    Corrected spots per well (CSPW mean peptide wells as compared to no antigen wells using 3.5 × 10 5 cells/well) was correlated by linear regression with tumor volume (mm 3 ) for animals that received vaccination with pooled epitopes to a specific antigens and animals that received PBS controls. IFN-g immune response was evaluated by IFN-g ELISPOT. a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6. R 2 and p value shown in each panel.
    Figure Legend Snippet: Corrected spots per well (CSPW mean peptide wells as compared to no antigen wells using 3.5 × 10 5 cells/well) was correlated by linear regression with tumor volume (mm 3 ) for animals that received vaccination with pooled epitopes to a specific antigens and animals that received PBS controls. IFN-g immune response was evaluated by IFN-g ELISPOT. a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6. R 2 and p value shown in each panel.

    Techniques Used: Enzyme-linked Immunospot

    Indirect ELISA of sera from women without breast atypia ( n = 43) was compared to sera from women undergoing breast surgery for mammographic abnormalities (benign pathology n = 12, hyperplasia n = 12, fibroadenoma n = 36, or DCIS n = 59), and women with known breast cancer ( n = 37). Y axis is ng/mL IgG antibody concentration by ELISA for each antigen and X axis is the breast pathology a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6 Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
    Figure Legend Snippet: Indirect ELISA of sera from women without breast atypia ( n = 43) was compared to sera from women undergoing breast surgery for mammographic abnormalities (benign pathology n = 12, hyperplasia n = 12, fibroadenoma n = 36, or DCIS n = 59), and women with known breast cancer ( n = 37). Y axis is ng/mL IgG antibody concentration by ELISA for each antigen and X axis is the breast pathology a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6 Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

    Techniques Used: Indirect ELISA, Concentration Assay, Enzyme-linked Immunosorbent Assay



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    Image Search Results


    Homology and function of selected antigens.

    Journal: NPJ Breast Cancer

    Article Title: Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients

    doi: 10.1038/s41523-021-00257-1

    Figure Lengend Snippet: Homology and function of selected antigens.

    Article Snippet: Positive controls were VPS35 goat polyclonal (Abnova ab10099 1:5000), ARPC2 goat polyclonal (Abnova PAB6168 1:1000), SERBP1 mouse monoclonal (Abnova H00026135, 1:25,000), KRT8 mouse monoclonal (Abnova MAB9946 1:25,000), and PDIA6 mouse monoclonal (H00010130-M04 Abnova 1:200).

    Techniques: Migration, Inhibition

    Four pooled siRNA knocked down expression of the targets VPS35 ( a , b ), ARPC2 ( c , d ), SERBP1 ( e , f ), KRT8 ( g , h ), and Pdia6 ( i , j ). Decreased expression of these proteins demonstrate decreased survival ( a , c , e , g , and i ) and increased apoptosis ( b , d , f , h , and j ) as compared to mock transfected in either triple negative (TNBC, HCC70) or HER2 positive (HER2, SKBR3) human breast cancer cell lines. Survival is measured as % survival compared to mock transfected cells and apoptosis is measured as fold increase compared to mock transfected calls. All studies are in triplicate. Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

    Journal: NPJ Breast Cancer

    Article Title: Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients

    doi: 10.1038/s41523-021-00257-1

    Figure Lengend Snippet: Four pooled siRNA knocked down expression of the targets VPS35 ( a , b ), ARPC2 ( c , d ), SERBP1 ( e , f ), KRT8 ( g , h ), and Pdia6 ( i , j ). Decreased expression of these proteins demonstrate decreased survival ( a , c , e , g , and i ) and increased apoptosis ( b , d , f , h , and j ) as compared to mock transfected in either triple negative (TNBC, HCC70) or HER2 positive (HER2, SKBR3) human breast cancer cell lines. Survival is measured as % survival compared to mock transfected cells and apoptosis is measured as fold increase compared to mock transfected calls. All studies are in triplicate. Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

    Article Snippet: Positive controls were VPS35 goat polyclonal (Abnova ab10099 1:5000), ARPC2 goat polyclonal (Abnova PAB6168 1:1000), SERBP1 mouse monoclonal (Abnova H00026135, 1:25,000), KRT8 mouse monoclonal (Abnova MAB9946 1:25,000), and PDIA6 mouse monoclonal (H00010130-M04 Abnova 1:200).

    Techniques: Expressing, Transfection

    Percent decreased tumor growth from TgMMTV-neu (light gray) and C3(1)Tag mice (dark gray) n = 8/group as compared to control PBS vaccinated mice (white). Mice were vaccinated with peptides from VPS35, ARPC2, SERBP1, KRT8, and PDIA6 or PBS with CFA/IFA adjuvant ~14 days for three times and then with 3 × 10 5 syngeneic mouse mammary tumor cells (MMC for TgMMTV-neu and M6 for C3(1)Tag) implanted. **** p < 0.0001.

    Journal: NPJ Breast Cancer

    Article Title: Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients

    doi: 10.1038/s41523-021-00257-1

    Figure Lengend Snippet: Percent decreased tumor growth from TgMMTV-neu (light gray) and C3(1)Tag mice (dark gray) n = 8/group as compared to control PBS vaccinated mice (white). Mice were vaccinated with peptides from VPS35, ARPC2, SERBP1, KRT8, and PDIA6 or PBS with CFA/IFA adjuvant ~14 days for three times and then with 3 × 10 5 syngeneic mouse mammary tumor cells (MMC for TgMMTV-neu and M6 for C3(1)Tag) implanted. **** p < 0.0001.

    Article Snippet: Positive controls were VPS35 goat polyclonal (Abnova ab10099 1:5000), ARPC2 goat polyclonal (Abnova PAB6168 1:1000), SERBP1 mouse monoclonal (Abnova H00026135, 1:25,000), KRT8 mouse monoclonal (Abnova MAB9946 1:25,000), and PDIA6 mouse monoclonal (H00010130-M04 Abnova 1:200).

    Techniques: Control, Adjuvant

    Corrected spots per well (CSPW mean peptide wells as compared to no antigen wells using 3.5 × 10 5 cells/well) was correlated by linear regression with tumor volume (mm 3 ) for animals that received vaccination with pooled epitopes to a specific antigens and animals that received PBS controls. IFN-g immune response was evaluated by IFN-g ELISPOT. a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6. R 2 and p value shown in each panel.

    Journal: NPJ Breast Cancer

    Article Title: Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients

    doi: 10.1038/s41523-021-00257-1

    Figure Lengend Snippet: Corrected spots per well (CSPW mean peptide wells as compared to no antigen wells using 3.5 × 10 5 cells/well) was correlated by linear regression with tumor volume (mm 3 ) for animals that received vaccination with pooled epitopes to a specific antigens and animals that received PBS controls. IFN-g immune response was evaluated by IFN-g ELISPOT. a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6. R 2 and p value shown in each panel.

    Article Snippet: Positive controls were VPS35 goat polyclonal (Abnova ab10099 1:5000), ARPC2 goat polyclonal (Abnova PAB6168 1:1000), SERBP1 mouse monoclonal (Abnova H00026135, 1:25,000), KRT8 mouse monoclonal (Abnova MAB9946 1:25,000), and PDIA6 mouse monoclonal (H00010130-M04 Abnova 1:200).

    Techniques: Enzyme-linked Immunospot

    Indirect ELISA of sera from women without breast atypia ( n = 43) was compared to sera from women undergoing breast surgery for mammographic abnormalities (benign pathology n = 12, hyperplasia n = 12, fibroadenoma n = 36, or DCIS n = 59), and women with known breast cancer ( n = 37). Y axis is ng/mL IgG antibody concentration by ELISA for each antigen and X axis is the breast pathology a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6 Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

    Journal: NPJ Breast Cancer

    Article Title: Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients

    doi: 10.1038/s41523-021-00257-1

    Figure Lengend Snippet: Indirect ELISA of sera from women without breast atypia ( n = 43) was compared to sera from women undergoing breast surgery for mammographic abnormalities (benign pathology n = 12, hyperplasia n = 12, fibroadenoma n = 36, or DCIS n = 59), and women with known breast cancer ( n = 37). Y axis is ng/mL IgG antibody concentration by ELISA for each antigen and X axis is the breast pathology a VPS35, b ARPC2, c SERBP1, d KRT8, and e PDIA6 Error bars are standard error of mean (SEM) **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

    Article Snippet: Positive controls were VPS35 goat polyclonal (Abnova ab10099 1:5000), ARPC2 goat polyclonal (Abnova PAB6168 1:1000), SERBP1 mouse monoclonal (Abnova H00026135, 1:25,000), KRT8 mouse monoclonal (Abnova MAB9946 1:25,000), and PDIA6 mouse monoclonal (H00010130-M04 Abnova 1:200).

    Techniques: Indirect ELISA, Concentration Assay, Enzyme-linked Immunosorbent Assay